The missile was launched on Sunday from the US Navy-controlled San Nicolas Island off the coast of Los Angeles, California.
"The test missile exited its ground mobile launcher and accurately impacted its target after more than 500 kilometers of flight," the Pentagon said in a statement.
"Data collected and lessons learned from this test will inform the Department of Defense's development of future intermediate-range capabilities."
While the missile was described as "conventionally configured," meaning not nuclear-equipped, the launch was a sign of Washington beefing up its nuclear war-fighting capabilities in the wake of the collapse of the Intermediate-Range Nuclear Forces Treaty on August 2.
The INF had banned all land-based missiles, conventional and nuclear, that could travel between 500 and 5,500 kilometers (310 and 3,400 miles), in an effort to abolish a class of nuclear arms deployed across Europe by the United States and the then-Soviet Union.