"The children of the Pakistan earthquake need you. Many are sick and injured. And now, the outbreak of disease poses an immediate and very real threat," the queen says in a public service announcement.
"It only takes five dollars to immunise a child and give him or her a new lease on life. Help us reach the four million children who need protection from disease."
The media campaign has been launched in co-operation with the UN International Children's Emergency Fund (Unicef) and the public service announcements were being aired on television stations carried by Arabsat and Euro Sat.
Queen Rania visited earthquake survivors in Muzaffarabad, the devastated capital of Azad Kashmir, last week as a representative of Unicef, and took a planeload of relief supplies.
"It is very urgent to intensify efforts for the rehabilitation of affected people," she told reporters during her visit.
Jordan was among the first countries to send aid to Pakistan in the aftermath of the 7.6 Richter scale earthquake that struck on October 8.
King Abdullah II visited Pakistan on October 14, the first foreign head of state to do so after the quake.
The desert Kingdom has already sent five planes of relief goods to its fellow Muslim country along with a 25-bed mobile hospital with a 50-member staff.
State-run Jordan television is running a telethon to collect donations.