Iran's pro-reform government has repeatedly said it would not allow groups to carry out such attacks and no Iranians are thought to have directly executed suicide bombings in Israel or elsewhere in recent years.
But the presence of President Mohammad Khatami's adviser on women's affairs and a representative of Supreme Leader Ayatollah Ali Khamenei leant some official backing to Wednesday's event.
In a dark hall decorated with pictures of female Palestinian suicide bombers, dozens of men and women queued to fill out registration forms.
"As a Muslim, it is my duty to sacrifice my life for oppressed Palestinian children," said Maryam Partovi, 31, a mother of two.
A banner hanging over the main entrance quoted Khamenei as saying: "Sacrificing oneself for religion and national interest is the height of honour and bravery."